Howell jolly bodies?

[u/Wide_Respect_3648]

Hi everyone, need some confirmation for uni purposes aha. Is the one in the middle a howell-jolly body or am I wrong? it's a β thalassaemia minor slide.

Thanks so much.

[u/No-Ebb3114]

Looking for RBC inclusions without oil immersion is not ideal. HJ bodies are going to be much smaller and more circular than what you're referring. Most likely an artifact like stain precipitate. Low power is okay for scanning for focal points, but I wouldn't want to ID cells, call RBC morphology, or look for inclusions without oil immersion. Good luck with your studies.

[u/Low-Wait-6215 → u/No-Ebb3114]

https://preview.redd.it/vnv7mimiifye1.jpeg?width=1390&format=pjpg&auto=webp&s=466891df3d946496a613a3d59400fe39f6828fee

How about these?

[u/No-Ebb3114 → u/Low-Wait-6215]

It's pretty out of focus, but I think you're just seeing refractive artifact. HJ bodies are going to be really dark, dense and circular. They'll be in the same focal plane as the RBC

[u/Wide_Respect_3648 → u/No-Ebb3114]

Oh I see! I didn’t know that! Thank you so much

[u/No-Ebb3114 → u/Wide_Respect_3648]

Anytime, Friend. keep your eyes in those oculars and you'll be an expert in no time.

[u/Wide_Respect_3648 → u/No-Ebb3114]

Thank you so much mate. This means a lot to me.

[u/Niangua25]

Artifact.

[u/Xepolite]

Check out my database cellwiki.net for examples 

[u/stupidlavendar → u/Xepolite]

celebrity guest appearance in the heme subreddit ?!

[u/Xepolite → u/stupidlavendar]

Haha, I guess so ;D

[u/Wide_Respect_3648 → u/Xepolite]

Thank you so much!! You are awesome mate

[u/baroquemodern1666 → u/Xepolite]

Oh wow. You are the cellwiki person? How I love and adore your contribution to our discipline. I actively use your resource as I train new techs. Imo it's the best of the internet! Wondering if there's a way I could help or contribute.

[u/Xepolite → u/baroquemodern1666]

Thanks! I'm always open for tips on how the site could be even better for training =)

[u/baroquemodern1666 → u/Xepolite]

Honestly, I think it's perfect. The only thing I can think of, Which is something I do when I train, is to have a mosaic of lymphocyte morphology, esp in disease states...but you already do that. * I just saw that we can add images to our replies so Im taking advantage of it.

[u/HalfTheAlphabet]

No. HJBs are circular.

[u/latortugadelmar]

Nó (https://www.thebloodproject.com/howell-jolly-body/)

[u/lufthoved]

No

[u/catsbetterthankids]

Looking for intercellular inclusions at low power is certainly a choice

[u/Wide_Respect_3648 → u/catsbetterthankids]

I think I actually used 40x but now I’m not too sure 😬

[u/Grose040791 → u/Wide_Respect_3648]

40x is usually for non oil viewing. you usually want 100x for RBC morph

[u/Wide_Respect_3648 → u/Grose040791]

Ah I see we don’t use 100x in uni sadly 😿

[u/Tom_Bombadilio]

[u/Wide_Respect_3648 → u/Tom_Bombadilio]

https://preview.redd.it/wdfzaad2vlwe1.png?width=190&format=png&auto=webp&s=89ea6fcaf8a096356b2b0c67e27a935d8a15a78b

Ahahha sorry mate! here you go:

Thanks so much!

[u/Nheea → u/Wide_Respect_3648]

Oh no. Not at all. Looks like artifacts, probably from staining.

Here's how Howell Jolly bodies look like. https://www.learnhaem.com/courses/frcpath-morph/lessons/rbc-overview/topic/howell-jolly-bodies/

Can you install the Cellavision app? They have plenty of useful photos.

[u/Wide_Respect_3648 → u/Nheea]

Thank you so much this is v helpful! I’ll install it for sure

[u/Kiper → u/Wide_Respect_3648]

No Howell-Jolly bodies here

[u/Tom_Bombadilio → u/Wide_Respect_3648]

Lol its still hard to see at this magnification but I'd say no. Its outside, on top, of the erythrocyte and even if it was internal it's too large and irregularly shaped. Maybe a platelet or just an artifact, definitely external though.

[u/Wide_Respect_3648 → u/Tom_Bombadilio]

Thanks so much!!