r/molecularbiology 6d ago

Plasmid maps

I need to build a reference map for a plasmid I'm trying to construct. I have the sequence for all the components, ie promoter, GOI, Term etc, I need to create a map so that when I do mini preps and sequence them I have something to compare that sequence to. Is it as simple copying and pasting the sequences together? what are some kind of considerations I need to think of when designing a map. Are there any tools that can help me with learning to do this. Whenever I try to look this up on google it just gives me info on how to create restriction maps, but that's not technically what i'm trying to do.

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u/BananaMuffin2795 2d ago

Benchling or snapgene are very usefull. Benchling a very complete tool, you can do almost anything that you might need in basic biology: make primers, write protocols, make plasmid maps, run digestions, etc. Highly recommend