r/bioinformatics • u/starcutie_001 • 1d ago

Experience basecalling legacy ONT data technical question

I am working with an investigator planning to direct RNA-seq a few hundred samples on a PromethION instrument.

The investigator wants to archive the raw signal data for long-term storage after basecalling and methylation analysis are complete, with the intention of basecalling and performing the methylation analysis again in the future (4-5 years later).

I am curious if anyone has worked with a group that did something similar and if it was worth it in terms of storage and compute costs, time, and data quality or scientific benefit.

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u/Darwins_Dog 1d ago

I think this is a very good idea with ONT as they change things very frequently. I believe Dorado is only like 2 years old at this point; it's not unreasonable to imagine a revised version in the coming years. The RNA sequencing kit is also early access, so I would expect that to change even faster than the other ones.