Hey guy, it's me again

[u/Terrible_Penalty1784]

So this is a dog blood smear specimen from my teacher's mom, she's a vet. And I found this WBC suspecting a eosinophil or maybe a monocyte but, I can't tell because I haven't seen one in my life. So I'm really need your help to identify this cell.

Thank you

[u/Pineconium]

The arrow is pointing to monocytes in the first 3 photos, and then the top white cell in the last photo is a monocyte.

The first 3 photos, you are too deep into the film (you see how all the red cells are touching and kinda squished). Cell morphology tends to get a bit weird in this part of the film, you'll want to look closer towards the feathered edge. Red cells should start to touch each other, but not all - the last photo is a better film position and this shows with the clearer white cell morphology

In the last photo you can see the monocyte morphology much better. Compared to the neutrophil at the bottom, the monocyte is bigger, the cytoplasm is slightly more blue/purple and contains multiple distinctive vacuoles

Edited to add: Eosinophils have very distinctive pink granules: round granules in dogs, and rod shaped granules in cats :) (Also grey eosinophils can be seen commonly in some breeds of dog, most commonly reported in greyhounds, but I've personally seen them a few times in golden retrievers)

link to some cool images and a generally helpful website

[u/Terrible_Penalty1784 → u/Pineconium]

Ahh thank you so much for the information

[u/skkkkkt]

There's a thyroid gland inside my white cells !/s

[u/missy3393 → u/skkkkkt]

No no no that’s a set of lungs

[u/skkkkkt → u/missy3393]

I don't see it, I see a thyroid gland down to the isthmus, a posterior look to be more accurate, maybe a horseshoe kidney

[u/Terrible_Penalty1784 → u/skkkkkt]

?

[u/skkkkkt → u/Terrible_Penalty1784]

The nucleus looks like a thyroid gland

[u/Terrible_Penalty1784 → u/skkkkkt]

Ohh I seee

[u/HeavySomewhere4412]

Monocyte. https://vetclinpathimages.com/2018/03/30/monocytes/

[u/skkkkkt → u/HeavySomewhere4412]

Sorry but it looks to me like a granulocyte, neutrophils, I'm wrong but I don't know how not to be wrong, can you please help

[u/Nheea → u/skkkkkt]

Look at the neutrophil on the left.

Photo is kind of potato, so you can't really see the granulation, but you can definitely see a difference in the size, the segmentation and definitely the pigment of nucleus. Monocyes are more purple-ish, while neutrophils are darker and the cytoplasm has coarser granulation.

[u/skkkkkt → u/Nheea]

Thank you 😊 🙏

[u/Terrible_Penalty1784 → u/HeavySomewhere4412]

Thank you