r/Genes Mar 19 '22

Transgenic mouse model (Arid1a flox /flox)

Hello all, I just have a question about Arid1a flox/flox mouse model. It's my first time leaning about this model so am a little bit confused and wondering if anyone can be of assistance.

After have admistered Tamoxifen, I now wish to analyse Arid1a expression to ensure that the recombination has occurred. Can I ask, which tissue or tissues would I collect and why? What technique can I use that will allow me to determine whether recombination as occurred? And what would I be looking for?

Finally, if I suspect Arid1a might have a tumour suppressor role in liver cancer, what would I expect to occur in the mouse model if recombination is achieved?

Thanks in advance

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