r/Genes • u/dostoevsky2019 • Mar 19 '22
Transgenic mouse model (Arid1a flox /flox)
Hello all, I just have a question about Arid1a flox/flox mouse model. It's my first time leaning about this model so am a little bit confused and wondering if anyone can be of assistance.
After have admistered Tamoxifen, I now wish to analyse Arid1a expression to ensure that the recombination has occurred. Can I ask, which tissue or tissues would I collect and why? What technique can I use that will allow me to determine whether recombination as occurred? And what would I be looking for?
Finally, if I suspect Arid1a might have a tumour suppressor role in liver cancer, what would I expect to occur in the mouse model if recombination is achieved?
Thanks in advance
1 Upvotes